Trypanosoma musculi Kendall (ATCC® 30181)

Organism: Trypanosoma musculi Kendall  /  Depositor: DG Dusanic

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Strain Designations Particino 2
Application
Vector borne research
Biosafety Level 1
Isolation
house mouse, Mus sp., Particino, Sicily, 1962
Product Format frozen
Type Strain no
Comments
Hemagglution technique to measure antibody
Kinetics of immunoglobulin M and G responses
Absorption of ablastic activity from mouse serum
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor DG Dusanic
Chain of Custody
ATCC <<--DG Dusanic<<--H.E. Krampitz
Year of Origin 1962
References

House RV, Dean JH. Adoptive cell transfer studies to examine the role of T lymphocytes in immunity to Trypanosoma musculi. J. Parasitol. 74: 819-827, 1988. PubMed: 2971101

Krampitz HE. [Geographical distribution, host-parasite relationship and multiplication of Sicilian strains of Trypanosoma (Herpetosoma) duttoni Thiroux 1950 (Protozoa, Trypanosomatidae)]. Z. Parasitenkd. 32: 297-315, 1969. PubMed: 4912259

Brooks BO, Reed ND. Trypanosoma musculi: Passive hemagglution technique to measure antibody in mice. Exp. Parasitol. 52: 49-52, 1981. PubMed: 7238725

Brooks BO, et al. Kinetics of immunoglobulin M and G responses of nude and normal mice to Trypanosoma musculi. Infect. Immun. 36: 667-671, 1982. PubMed: 6979515

Brooks BO, Reed ND. Absorption of ablastic activity from mouse serum by using a Trypanosoma musculi population rich in dividing forms. Infect. Immun. 27: 94-96, 1980. PubMed: 7358433

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation