Trypanosoma cruzi Chagas (ATCC® 30161)

Organism: Trypanosoma cruzi Chagas  /  Depositor: RG Yaeger

Permits and Restrictions

View Permits

Strain Designations Houston
Application
Vector borne research
Biosafety Level 2
Isolation
6-month-old boy, Houston, TX, 1955
Product Format frozen
Type Strain no
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor RG Yaeger
Chain of Custody
ATCC <<--RG Yaeger<<--Texas State Hlth. Dept
Year of Origin 1955
References

National Office of Vital Statistics: Communicable Diseases Summary for Week Ending December 10, 1955. : U.S. Dept. HEW; .

Proc. 6th Int. Cong. Trop. Med. Malaria 3: 158, 1958.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

Basic Documentation