Leishmania mexicana (Biagi) Garnham (ATCC® 30031)

Strain Designations: Guatemalan  /  Depositor: EJ Tobie  /  Biosafety Level: 2

Permits and Restrictions

View Permits

Deposited As Leishmania braziliensis Vianna
Strain Designations Guatemalan
Application
Vector borne research
Biosafety Level 2
Isolation Human, Guatemala, 1948
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Comments
Promastigotes
Lipids
Antigenic differentiation
Originally deposited as Leishmania braziliensis. Re-classified as Leishmania mexicana based on ITS1, 5.8S rRNA, and ITS2 sequencing performed at ATCC.
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).  The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1ºC freezing apparatus.  Place the apparatus at -80ºC for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh tube of ATCC medium 1011. 
  10. Incubate vertically at 25°C with the cap screwed on tightly.
  11. Maintain as described above. 
Name of Depositor EJ Tobie
Year of Origin 1948
References

Beach DH, et al. Lipids of Leishmania promastigotes. J. Parasitol. 65: 201-216, 1979. PubMed: 448607

Constantine NT, Anthony RL. Antigenic differentiation of the kinetoplastids Leishmania braziliensis and Trypanosoma cruzi by means of monoclonal antibodies. J. Protozool. 30: 346-350, 1983. PubMed: 6195336

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

Basic Documentation