Tritrichomonas foetus (Riedmuller) Wenrich and Emerson (ATCC® 30003)

Organism: Tritrichomonas foetus (Riedmuller) Wenrich and Emerson  /  Depositor: LS Diamond

Permits and Restrictions

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Strain Designations BP-4: Beltsville
Biosafety Level 2
Isolation Preputial washings of a bull, Bos bovis, Beltsville, MD, 1956
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain no
Comments
Monoclonal antibodies with effector function
Comparison of isolates virulence
Use of plastic ampoules for freeze preservation
This strain of T. foetus may be cultured over an unusually wide temperature range. The growth rate will be faster at 35°C than at 25°C.
Medium ATCC® Medium 2154: LYI Entamoeba medium
ATCC® Medium 359: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 7.2 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use
Growth Conditions
Temperature: 25°C to 37°C
Atmosphere: Anaerobic
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. When cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.
  2. Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.
    1. Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube;
    2. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium;
    3. Invert several times to dissolve the DMSO;
    4. Allow to warm to room temperature.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.From room temperature cool at -1°C/min to -40°C.If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.(The cooling rate in this apparatus is approximately -1°C/min.)
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 359 (completed with serum) or 13 mL ATCC Medium 2154.
  10. Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154).
Name of Depositor LS Diamond
Year of Origin 1956
References

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

Burgess DE. Tritrichomonas foetus: preparation of monoclonal antibodies with effector function. Exp. Parasitol. 62: 266-274, 1986. PubMed: 2427356

Hook RR J, et al. Tritrichomonas foetus: comparison of isolate virulence in an estrogenized mouse model. Exp. Parasitol. 81: 202-207, 1995. PubMed: 7556562

Felleisen RS, et al. Detection of Tritrichomonas foetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences. J. Clin. Microbiol. 36: 513-519, 1998. PubMed: 9466768

Felleisen RS. Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique. Parasitol. Res. 84: 153-156, 1998. PubMed: 9493217

Felleisen RS. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa. Parasitology 115: 111-119, 1997. PubMed: 10190167

Bouma MJ, et al. Activity of disulfiram (bis(diethylthiocarbamoyl)disulphide) and ditiocarb (diethyldithiocarbamate) against metronidazole-sensitive and -resistant Trichomonas vaginalis and Tritrichomonas foetus. J. Antimicrob. Chemother. 42: 817-820, 1998. PubMed: 10052908

Babal P, et al. Sialic acid-specific lectin from Tritrichomonas foetus. Biochim. Biophys. Acta 1428: 106-116, 1999. PubMed: 10366765

Wei-Dong Xu, et al. Chromosome numbers of Tritrichomonas foetus and Tritrichomonas suis. Vet. Parasitol. 78: 247-251, 1998. PubMed: 9786624

Lun Z-R, et al. Comparison of growth rates of Tritrichomonas foetus isolates from various geographic regions using three different culture media. Vet. Parasitol. 89: 199-208, 2000. PubMed: 10760410

Kennett MJ, Hook RR Jr.. Tritrichomonas foetus: characterization of isolates and partial purification of a secreted cytotoxin. Exp. Parasitol. 102: 1-8, 2002. PubMed: 12615161

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
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