Cercomonas plasmodialis Mylnikov (ATCC® 50418)

Strain Designations: AZ-6  /  Depositor: TK Sawyer  /  Biosafety Level: 1

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Strain Designations AZ-6
Application
This is the only species of this genus that produces a microplasmodium.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
plant
Product Format dried
Type Strain no
Comments
This is the only species of this genus that produces a microplasmodium. The microplasmodium gives rise to large multinucleate cysts.
dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992.
Medium ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria
Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802.
Subcultivation
Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802.
Name of Depositor TK Sawyer
Chain of Custody
ATCC <<--TK Sawyer<<--T.A. Nerad
References

dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation