Willaertia magna De Jonckheere et al. (ATCC® 50035)

Organism: Willaertia magna De Jonckheere et al.  /  Depositor: JF De Jonckheere

Permits and Restrictions

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Strain Designations Z503
Biosafety Level 1
Isolation
bovine feces, France
Product Format frozen
Type Strain yes
Comments
Isoenzyme electrophoresis
Occurrence in natural waters in Mexico
review
Medium ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Growth Conditions
Temperature: 35.0°C
Duration: axenic
Cryopreservation
1.   Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.

2.   Adjust the concentration of cells to 2.0 x 106/ml.  If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.

3.   Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:  Add the required volume of DMSO to a glass screw-capped test tube and place on ice.  Allow the DMSO to solidify.  Add the required volume of refrigerated ATCC medium 1034.  Dissolve the DMSO by inverting several times.  If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.

4.   Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 60 min.

5.   Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.   Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.

7.   The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8.   To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.

9.   Immediately after thawing, do not leave in the water bath. Aseptically remove the contents of the ampule and inoculate into a fresh tube or flask of ATCC medium 1034.

10.          Incubate at 35°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).

Name of Depositor JF De Jonckheere
Chain of Custody
ATCC <<--JF De Jonckheere<<--M. Pussard
References

Pussard M, Pons R. Etude des pores kystiques de Naegleria (vahlkampfiidae - amoebida). Protistologica 15: 163-175, 1979.

Environ. Res. 59: 223-226, 1992.

De Jonckheere DG, et al. Willaertia magna gen. nov., sp. nov. (Vahlkampfiidae), a thermophilic amoeba found in different habitats. Protistologica 20: 5-13, 1984.

John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993

De Jonckheere JF. Variation of electrophoretic karyotypes among Naegleria spp.. Parasitol. Res. 76: 55-62, 1989. PubMed: 2622896

Rivera F, et al. Pathogenic amoebae in natural thermal waters of three resorts of Hidalgo, Mexico. Environ. Res. 50: 289-295, 1989. PubMed: 2583075

Rivera F, et al. Contaminacion del liquido cefolorraquideo de un infante con sindrome de Arnold-Chiari tipo II, hidrocefalia y mielomeningocele, por Naegleria lovaniensis. Rev. Enferm. Infecc. Pediatr. 2: 91-94, 1989.

type strain

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation