Leishmania tropica (Wright) Luhe (ATCC® 30816)

Strain Designations: WHO Israel LRC 146  /  Depositor: J Janovy  /  Biosafety Level: 2

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Deposited As Leishmania adleri
Strain Designations WHO Israel LRC 146
Application
Vector borne research
Biosafety Level 2
Isolation
lizard, Latastia longicauda revoili, Kenya
Product Format frozen
Type Strain no
Comments
Taxonomy (ATCC lab.). Promastigotes.
In vitro phagocytosis by lizard leukocytes
Taxonomic status
Variation in kinetoplast minicircle and maxicircle DNA sequences
Cyclopropane fatty acid
Medium Medium 811: Leishmania medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor J Janovy
Chain of Custody
ATCC <<--J Janovy<<--WHO LRC Jerusalem <<--- . . . <<--- Heisch?
References

Dollahon NR, Janovy J Jr.. Leishmania adleri: in vitro phagocytosis by lizard leukocytes and peritoneal exudate cells. Exp. Parasitol. 34: 56-61, 1973. PubMed: 4722485

Dollahon NR, Janovy J Jr.. Experimental infection of New World lizards with Old World lizard Leishmania species. Exp. Parasitol. 36: 253-260, 1974. PubMed: 4413377

Simpson L. The status of Leishmania tarentolae/Trypanosoma platydactyli. Parasitol. Today 4: 115-118, 1988.

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation