Tetrahymena furgasoni Nanney and McCoy (ATCC® 10542)

Organism: Tetrahymena furgasoni Nanney and McCoy  /  Depositor: RP Hall

Permits and Restrictions

View Permits

Deposited As Tetrahymena geleii Furgason
Strain Designations W [SA13]
Application
assay of arginine L-arginine
assay of histidine L-histidine
assay of lysine L-lysine
assay of methionine
assay of protein
Biosafety Level 1
Isolation
Woods Hole, MA, 1939
Product Format test tube
Type Strain no
Comments
species description
magnetic fettering
effect of molecular weight of protein on growth
isoenzymic characterization of three mating groups
bioassays of protein quality
Osmoregulation
food for suctorians
electrophoretic characterization
Medium ATCC® Medium 357: Tetrahymena medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Cryopreservation
1.  Transfer Tetrahymena from usual growth medium to ATCC Medium 1034 and allow to grow to near peak density.

2.   Harvest cells from a culture by centrifugation at 300 x g for 2 min.          

3.   Adjust concentration of cells to 2 x 106/ml in fresh

      medium.

4.   While cells are centrifuging, prepare a 22% (v/v) sterile

solution of sterile DMSO in fresh medium.

a) Add 2.2 ml of DMSO to an ice cold 20 x 150 mm screw-capped test tube;

b) Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 7.8 ml of ice cold medium;

c) Invert several times to dissolve the DMSO;

d) Allow to warm to room temperature.

5.   Add a volume of the DMSO solution equal to the cell

      suspension volume but add in 3 equal aliquots at 2 min

      intervals. Thus, the final concentration of the preparation

      will equal 11% (v/v) DMSO and 106 cells /ml.

6.   Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic

      screw-capped cryules (special plastic vials for       cryopreservation).

7.   Place the ampules in a controlled rate freezing unit. The

cooling cycle should be initiated no less than 15 min and no longer than 60 min after the addition of the DMSO to the cell preparation. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At  -50°C ampules are plunged into liquid nitrogen.

8.   Store in the vapor or liquid phase of a nitrogen

      refrigerator.

9.   To establish a culture from the frozen state aseptically add 0.5 ml of sterile modified PYNFH medium (ATCC Medium 1034) containing 8% (w/v) sucrose to the ampule.  Immediately, place in a 35°C water bath, until thawed. Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule.

10.          Immediately after thawing, aseptically remove the contents of the ampule and gently add the material to the edge of a 20 x 100 mm Petri plate containing ATCC Medium 919 (non-nutrient agar) and position on a 15 degree slant.  The cell suspension will pool at the edge of the plate.

11.          Continue to double the volume of the cell suspension at 10

minute intervals by adding ATCC medium 1034) containing 4% sucrose (w/v).  When the volume reaches 16.0 ml place the plate in horizontal position and incubate at 25°C. 

12.          On the following day, gently remove the cell suspension for the plate and transfer to a T-25 tissue culture flask.  Note the volume of the suspension and add a volume of fresh medium containing 4% sucrose equal to the volume of  the cell suspension.  Incubate the culture at 25°C.

13.          After culture has been established subculture into fresh

      normal medium without sucrose. 

Name of Depositor RP Hall
Chain of Custody
ATCC <<--RP Hall<<--M.S. Dunn strain W (amicronucleate) (1948) <<--- G.W. Kidder <<--- . . . <<--- C.L. Claff)
Year of Origin 1939
References

Borden D, et al. Electrophoretic characterization of classical Tetrahymena pyriformis strains. J. Protozool. 20: 693-700, 1973. PubMed: 4148695

Biol. Bull. 78: 9-23, 1940.

Williams NE, et al. Protein similarities in the genus Tetrahymena and a description of Tetrahymena leucophrys n. sp.. J. Protozool. 31: 313-321, 1984.

Font R, Ballentine R. Magnetic fettering of the ciliated protozoan Tetrahymena pyriformis. Trans. Am. Microsc. Soc. 95: 189-197, 1976.

Hunter B, et al. Effect of molecular weight of protein on growth of Tetrahymena pyriformis. Nutr. Rep. Int. 24: 1043-1055, 1981.

Nanney DL, et al. Isoenzymic characterization of three mating groups of the Tetrahymena pyriformis complex. J. Protozool. 27: 451-459, 1980.

Rifkin JL. The role of the contractile vacuole in the osmoregulation of Tetrahymena pyriformis. J. Protozool. 20: 108-114, 1973. PubMed: 4347868

Henk WG, Paulin JJ. Scanning electron microscopy of budding and metamorphosis in Discophrya collini Toort. J. Protozool. 24: 134-139, 1977. PubMed: 405477

Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037

Stott JA, Smith H. Microbiological assay of protein quality with Tetrahymena pyriformis W. Br. J. Nutr. 20: 663-673, 1966. PubMed: 5957407

Dryden MJ, et al. Predicting protein digestibility and quality using an enzyme-Tetrahymena pyriformis W bioassay. J. Food Biochem. 1: 35-44, 1977.

Evancho GM, et al. Comparison of Tetrahymena pyriformis W and rat bioassays for the determination of protein quality. J. Food Sci. 42: 444-448, 1977.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation