Lobosphaera sp. (ATCC® 75630)

Strain Designations: TM-33  /  Depositor: San-Ei Gen F.F.I., Inc.  /  Biosafety Level: 1

Permits and Restrictions

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Strain Designations TM-33
Application
produces trehalase
Biosafety Level 1
Isolation
soil, Yao City, Osaka Pref., Japan
Product Format frozen
Type Strain no
Medium Medium 2027: Lobosphaera medium
Growth Conditions
Max Temperature: 35.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 75630 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5.0 ml of ATCC medium 2027 in a 16 x 125 mm screw-capped test tube. Incubate on a 15 degree horizontal slant at 25-35C in the dark. Cultivation of the strain in the dark with agitation will increase the growth rate and the yield of trehalase (see patent for more information).
Subcultivation
Protocol: ATCCNO: 75630 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5.0 ml of ATCC medium 2027 in a 16 x 125 mm screw-capped test tube. Incubate on a 15 degree horizontal slant at 25-35C in the dark. Cultivation of the strain in the dark with agitation will increase the growth rate and the yield of trehalase (see patent for more information).
Name of Depositor San-Ei Gen F.F.I., Inc.
References

Kitahata S, et al. Alga species lobsphaera TM-33 (ATCC 75630) which is useful for preparing trehalase. US Patent 5,529,927 dated Jun 25 1996

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation