Lobosphaera sp. (ATCC® 75630)

Organism: Lobosphaera sp.  /  Depositor: San-Ei Gen F.F.I., Inc.

Strain Designations TM-33
Application
produces trehalase
Biosafety Level 1
Isolation
soil, Yao City, Osaka Pref., Japan
Product Format frozen
Type Strain no
Medium Medium 2027: Lobosphaera medium
Growth Conditions
Max Temperature: 35.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 75630 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5.0 ml of ATCC medium 2027 in a 16 x 125 mm screw-capped test tube. Incubate on a 15 degree horizontal slant at 25-35C in the dark. Cultivation of the strain in the dark with agitation will increase the growth rate and the yield of trehalase (see patent for more information).
Subcultivation
Protocol: ATCCNO: 75630 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5.0 ml of ATCC medium 2027 in a 16 x 125 mm screw-capped test tube. Incubate on a 15 degree horizontal slant at 25-35C in the dark. Cultivation of the strain in the dark with agitation will increase the growth rate and the yield of trehalase (see patent for more information).
Name of Depositor San-Ei Gen F.F.I., Inc.
References

Kitahata S, et al. Alga species lobsphaera TM-33 (ATCC 75630) which is useful for preparing trehalase. US Patent 5,529,927 dated Jun 25 1996