Lobosphaera sp. (ATCC® 75630™) Organism: Lobosphaera sp. / Depositor: San-Ei Gen F.F.I., Inc. General Information Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Strain Designations TM-33 Application produces trehalase Biosafety Level 1 Isolation soil, Yao City, Osaka Pref., Japan Product Format frozen Type Strain no Medium ATCC® Medium 2027: Lobosphaera medium Growth Conditions Max Temperature: 35.0°CMin Temperature: 25.0°CDuration: axenicProtocol: ATCCNO: 75630 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5.0 ml of ATCC medium 2027 in a 16 x 125 mm screw-capped test tube. Incubate on a 15 degree horizontal slant at 25-35C in the dark. Cultivation of the strain in the dark with agitation will increase the growth rate and the yield of trehalase (see patent for more information). Subcultivation Protocol: ATCCNO: 75630 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5.0 ml of ATCC medium 2027 in a 16 x 125 mm screw-capped test tube. Incubate on a 15 degree horizontal slant at 25-35C in the dark. Cultivation of the strain in the dark with agitation will increase the growth rate and the yield of trehalase (see patent for more information). Name of Depositor San-Ei Gen F.F.I., Inc. References Kitahata S, et al. Alga species lobsphaera TM-33 (ATCC 75630) which is useful for preparing trehalase. US Patent 5,529,927 dated Jun 25 1996 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS