Polytoma difficile Pringsheim (ATCC® 50459)

Organism: Polytoma difficile Pringsheim  /  Depositor: CW Birky

Strain Designations SAG 62-16 sc1
Biosafety Level 1
Isolation
clone derived from strain SAG 62-16
Product Format frozen
Type Strain no
Genome Sequenced Strain

Yes

Comments
The SSU rRNA gene of this strain has been sequenced.
Medium ATCC® Medium 1900: Soil extract salts medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 50458 SPEC: After thawing the frozen material transfer contents to 10 ml of fresh medium in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 25C. Subculture every 14 days by agitating the flask and aseptically transferring a 0.1-ml aliquot to a fresh flask of medium.
Subcultivation
Protocol: ATCCNO: 50458 SPEC: After thawing the frozen material transfer contents to 10 ml of fresh medium in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 25C. Subculture every 14 days by agitating the flask and aseptically transferring a 0.1-ml aliquot to a fresh flask of medium.
Name of Depositor CW Birky
Chain of Custody
ATCC <<--CW Birky<<--SAG