Neospongiococcum excentricum Deason and Bold (ATCC® 40335™)

Organism: Neospongiococcum excentricum Deason and Bold  /  Depositor: Universal Foods Corp.

Strain Designations HS1 [B]
Application
produces carotenoids
Biosafety Level 1
Isolation
division mutant derived from wild type strain UTEX 1232, 1987
Product Format frozen
Type Strain no
Comments
Carotenoid producing culture
Medium ATCC® Medium 2001: CYU 2%
Growth Conditions
Max Temperature: 35.0°C
Min Temperature: 25.0°C
Protocol: ATCCNO: 40335 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 2001 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen the cap one half turn, and incubate the flask on a horizontal slant (5-15 degree slant) at 25-35C. The culture should be ready to subculture in 5-7 days. To subculture, screw the cap on tightly, agitate vigorously and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2001. Incubate as above.
Subcultivation
Protocol: ATCCNO: 40335 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 2001 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen the cap one half turn, and incubate the flask on a horizontal slant (5-15 degree slant) at 25-35C. The culture should be ready to subculture in 5-7 days. To subculture, screw the cap on tightly, agitate vigorously and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2001. Incubate as above.
Name of Depositor Universal Foods Corp.
Chain of Custody
ATCC <<--Universal Foods Corp.<<--D.L. Heefner
References

Liao HH, et al. Carotenoid producing culture using Nespongiococcum excentricum. US Patent 5,437,997 dated Aug 1 1995

Orndorff SA, et al. Zeaxanthin producing strains of Neospongiococcum excentricum. US Patent 5,360,730 dated Nov 1 1994