Euglena gracilis Klebs (ATCC® 12716)

Strain Designations: Z  /  Depositor: SH Hutner  /  Biosafety Level: 1

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Strain Designations Z
Application
assay of vitamin B12 cobalamin, cobalamins
Biosafety Level 1
Isolation
freshwater material from Saedeleer, 1950
Product Format frozen
Type Strain no
Comments
Medium for Vitamin B12 assay
Regulation of cell shape
Growth and cell volume
Aromatic amino acid pathway
water-soluble vitamins in cells and spent culture supernatants
Molecular biology
Synchronization of division
cryopreservation
photosynthetic
Medium Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Cryopreservation

1.   Harvest cells from a culture which is at or near peak density by centrifuging at 100 x g for 1 minute.

Name of Depositor SH Hutner
Chain of Custody
ATCC <<--SH Hutner<<--E.G. Pringsheim
Year of Origin 1950
References

Byng GS, et al. The aromatic amino acid pathway branches at L-arogenate in Euglena gracilis. Mol. Cell. Biol. 1: 426-438, 1981. PubMed: 6152855

Shehata TE, Kempner ES. Growth and cell volume of Euglena gracilis in different media. Appl. Environ. Microbiol. 33: 874-877, 1977. PubMed: 405924

Hutner SH, et al. A sugar-containing basal medium for vitamin B12-Assay with Euglena; Application to body fluids. J. Protozool. 3: 101-112, 1956.

Lonergan TA, Williamson LC. Regulation of cell shape in Euglena gracilis. V. Time-dependent responses to Ca2+ agonists and antagonists. J. Cell Sci. 89: 365-371, 1988. PubMed: 3143739

Analytical microbiology. New York: Academic Press; 1963.

Analytical microbiology. New York: Academic Press; 1963.

Hwang SW, Hudock GA. Stability of Chlamydomonas reinhardi in liquid nitrogen storage. J. Phycol. 7: 300-303, 1971.

Hwang SW, Horneland W. Survival of algal cultures after freezing by controlled and uncontrolled cooling. Cryobiology 1: 305-311, 1965. PubMed: 5872223

Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981.

Kempner ES, Miller JH. The molecular biology of Euglena gracilis. IX. Amino acid pool composition. J. Protozool. 21: 363-367, 1974. PubMed: 4209142

Shehata TE, Kempner ES. Synchronization of division in vitamin B12-starved Euglena gracilis. J. Protozool. 26: 626-630, 1979. PubMed: 120896

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation