Euglena gracilis Klebs (ATCC® 12716)

Strain Designations: Z  /  Depositor: SH Hutner  /  Biosafety Level: 1

Permits and Restrictions

View Permits

Strain Designations Z
assay of vitamin B12 cobalamin, cobalamins
Biosafety Level 1
freshwater material from Saedeleer, 1950
Product Format frozen
Type Strain no
Medium for Vitamin B12 assay
Regulation of cell shape
Growth and cell volume
Aromatic amino acid pathway
water-soluble vitamins in cells and spent culture supernatants
Molecular biology
Synchronization of division
Medium ATCC® Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 25.0°C
Duration: axenic

1.   Harvest cells from a culture which is at or near peak density by centrifuging at 100 x g for 1 minute.

Name of Depositor SH Hutner
Chain of Custody
ATCC <<--SH Hutner<<--E.G. Pringsheim
Year of Origin 1950

Byng GS, et al. The aromatic amino acid pathway branches at L-arogenate in Euglena gracilis. Mol. Cell. Biol. 1: 426-438, 1981. PubMed: 6152855

Shehata TE, Kempner ES. Growth and cell volume of Euglena gracilis in different media. Appl. Environ. Microbiol. 33: 874-877, 1977. PubMed: 405924

Hutner SH, et al. A sugar-containing basal medium for vitamin B12-Assay with Euglena; Application to body fluids. J. Protozool. 3: 101-112, 1956.

Lonergan TA, Williamson LC. Regulation of cell shape in Euglena gracilis. V. Time-dependent responses to Ca2+ agonists and antagonists. J. Cell Sci. 89: 365-371, 1988. PubMed: 3143739

Analytical microbiology. New York: Academic Press; 1963.

Analytical microbiology. New York: Academic Press; 1963.

Hwang SW, Hudock GA. Stability of Chlamydomonas reinhardi in liquid nitrogen storage. J. Phycol. 7: 300-303, 1971.

Hwang SW, Horneland W. Survival of algal cultures after freezing by controlled and uncontrolled cooling. Cryobiology 1: 305-311, 1965. PubMed: 5872223

Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981.

Kempner ES, Miller JH. The molecular biology of Euglena gracilis. IX. Amino acid pool composition. J. Protozool. 21: 363-367, 1974. PubMed: 4209142

Shehata TE, Kempner ES. Synchronization of division in vitamin B12-starved Euglena gracilis. J. Protozool. 26: 626-630, 1979. PubMed: 120896

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation