pSR663 (ATCC® MBA-28)

Applications: expression vectorvector component of two-hybrid screening system  /  Depositors: DA Daines

Permits and Restrictions

View Permits

Designations pSR663
Depositors DA Daines
Biosafety Level 1
Vector Information
Vector: pSR663 (plasmid)
Type: expression
Construction: pDP804, pTrcHisC
Features: marker(s): ampR
operator: mutant LexA
other: reading frame C
promoter: sulA
replicon: p15A
Applications
expression vector
vector component of two-hybrid screening system
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Daines DA, et al. Use of a LexA-based system to identify protein:protein interactions in vivo. Methods Enzymol. 358: 153-161, 2002. PubMed: 12474385

Daines DA, Silver RP. Evidence for multimerization of neu proteins involved in polysialic acid synthesis in Escherichia coli K1 using improved LexA-based vectors. J. Bacteriol. 182: 5267-5270, 2000. PubMed: 10960115

Related Products
component of:ATCC MBA-29
Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet