pSR663 (ATCC® MBA-28)

Applications: expression vectorvector component of two-hybrid screening system  /  Depositors: DA Daines

Permits and Restrictions

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Designations pSR663
Depositors DA Daines
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Vector: pSR663 (plasmid)
Type: expression
Construction: pDP804, pTrcHisC
Features: marker(s): ampR
operator: mutant LexA
other: reading frame C
promoter: sulA
replicon: p15A
expression vector
vector component of two-hybrid screening system
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Daines DA, et al. Use of a LexA-based system to identify protein:protein interactions in vivo. Methods Enzymol. 358: 153-161, 2002. PubMed: 12474385

Daines DA, Silver RP. Evidence for multimerization of neu proteins involved in polysialic acid synthesis in Escherichia coli K1 using improved LexA-based vectors. J. Bacteriol. 182: 5267-5270, 2000. PubMed: 10960115

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component of:ATCC MBA-29
Shipped freeze-dried