pFB-neo-CAMYEL plasmid in E. coli (ATCC® MBA-278)

Applications: It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo.  /  Depositors: L Jiang

Permits and Restrictions

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Designations pFB-neo-CAMYEL plasmid in E. coli
Depositors L Jiang
Biosafety Level 1
Vector Information
Size (kb): 10.4700000000000000
Vector: pFB-neo-CAMYEL plasmid in E. coli
Promoters: Eukaryotic Promoter for expression Mo-MLV LTR
Marker(s):neoR,AmpR
Features: eukaryotic selectable marker cassette: neoR
replicon: pMB1
transcription terminator: 3' LTR
Applications
It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo.
Comments
A retroviral vector for introducing the CAMYEL (cAMP sensor using YFP-Epac-RLuc) into mammalian cells. It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo. Vector features: 5'LTR: 209-760; viral packaging signal: 760-2046; gag gene: 1236-1723; CAMYEL: 2075-5977; IRES: 5992-6485; Neomycin resistance: 6662-7456; 3'LTR: 7516-8109; Amp resistance: 9408-10265. More information on this construct may be found at the AfCS website:http://www.signaling-gateway.org/data/plasmid/
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jiang LI, et al. Use of cAMP BRET sensor to characterize a novel regulation of cAMP by sphingosine-1-phosphate/G13 pathway. J. Biol. Chem. 282: 10576-10584, 2007.

Shipped frozen
Shipping Information  Frozen glycerol stock of E. coli containing the plasmid
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