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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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pFB-neo-CAMYEL plasmid in E. coli
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Vector Information
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Size (kb): 10.4700000000000000 Vector: pFB-neo-CAMYEL plasmid in E. coli Promoters: Eukaryotic Promoter for expression Mo-MLV LTR Marker(s):neoR,AmpR Features: eukaryotic selectable marker cassette: neoR
replicon: pMB1
transcription terminator: 3' LTR
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Applications
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It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo.
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Comments
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A retroviral vector for introducing the CAMYEL (cAMP sensor using YFP-Epac-RLuc) into mammalian cells. It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo.
Vector features: 5'LTR: 209-760; viral packaging signal: 760-2046; gag gene: 1236-1723; CAMYEL: 2075-5977; IRES: 5992-6485; Neomycin resistance: 6662-7456; 3'LTR: 7516-8109; Amp resistance: 9408-10265.
More information on this construct may be found at the AfCS website:http://www.signaling-gateway.org/data/plasmid/
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References
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Jiang LI, et al. Use of cAMP BRET sensor to characterize a novel regulation of cAMP by sphingosine-1-phosphate/G13 pathway. J. Biol. Chem. 282: 10576-10584, 2007.
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Shipping Information
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Frozen glycerol stock of E. coli containing the plasmid
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