pGEV-TRP1 plasmid in E. coli (ATCC® MBA-173)

Permits and Restrictions

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Designations pGEV-TRP1 plasmid in E. coli
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.4450000000000000
Vector: pGEV-TRP1 plasmid in E. coli (plasmid)
Type: expression
Promoters: Eukaryotic Promoter for expression MRP7
Construction: pRS314, MRP7 promoter, GEV (Gal4, B-estradiol activator, VP16)
Marker(s):AmpR
Comments
A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS314. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.