pRS307 (ATCC® MBA-13)

Applications: YI-type (integrating) shuttle vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pRS307
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 8.2200000000000000
Vector: pRS307 (plasmid)
Type: cloning
Construction: pUC
Construct size (kb): 8.2200003
Features: eukaryotic selectable marker cassette: LYS2
insert detection: LacZ
Applications
YI-type (integrating) shuttle vector
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Shipped frozen
Shipping Information

Shipped: Freeze dried Escherichia coli DH5 alpha containing the plasmid.

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