pRS307 (ATCC® MBA-13)

Applications: YI-type (integrating) shuttle vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pRS307
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.2200000000000000
Vector: pRS307 (plasmid)
Type: cloning
Construction: pUC
Construct size (kb): 8.2200003
Features: eukaryotic selectable marker cassette: LYS2
insert detection: LacZ
Applications
YI-type (integrating) shuttle vector
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Shipped frozen
Shipping Information

Shipped: Freeze dried Escherichia coli DH5 alpha containing the plasmid.

Product Sheet
Product Sheet
Product Sheet