pFa6a-TRP-pADH1 (ATCC® 87810)

Applications: cloning vector  /  Depositors: DJ DeMarini

Permits and Restrictions

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Designations pFa6a-TRP-pADH1
Depositors DJ DeMarini
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.1250000000000000
Vector: pFa6a-TRP-pADH1 (plasmid)
Promoters: Promoter ADH1
Construction: pCRTOPO2.1
Marker(s):ampR
Construct size (kb): 4.125
Features: marker(s): ampR, TRP1
promoter: ADH1
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--4.1; EcoRI--4.1; BglII/EcoRI--3.2, 0.9.
The marker and promoter are flanked by the F4 primer site(5') and the R6 primer site (3'). The size of the the F4/R6 PCR product should be 1482nt.
F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.
Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.
Growth Conditions
Temperature: 37.0°C
References

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899

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