HO-poly-KanMX4-HO (ATCC® 87804)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations HO-poly-KanMX4-HO
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 6.0599999427795410
Vector: HO-poly-KanMX4-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s):ampR
Construct size (kb): 6.059999942779541
Features: promoter: HO
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--3.0 (doublet); XbaI--6.0; PstI--5.8, 0.2.
The plasmid contains two fragments from the HO promoter inserted in pUC21-NotI polylinker along with the kanMX selectable marker. It allows for targeted integration at HO locus.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)
References

David J Stillman, personal communication

Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet