pUC21-NotI (ATCC® 87802)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

View Permits

Designations pUC21-NotI
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.2500000000000000
Vector: pUC21-NotI (plasmid)
Construction: pUC21deltaBB and pFA6b
Construct size (kb): 3.25
cloning vector
Restriction digests of the clone give the following sizes (kb): PvuI--2.0, 1.2; PstI--3.25; XbaI--3.25.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518

Shipped freeze-dried