pUC21-NotI (ATCC® 87802)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pUC21-NotI
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.2500000000000000
Vector: pUC21-NotI (plasmid)
Construction: pUC21deltaBB and pFA6b
Marker(s):ampR
Construct size (kb): 3.25
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuI--2.0, 1.2; PstI--3.25; XbaI--3.25.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)
References

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet