pUC21deltaBB (ATCC® 87801)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pUC21deltaBB
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 3.0999999046325680
Vector: pUC21deltaBB (plasmid)
Construction: pUC
Marker(s):ampR
Construct size (kb): 3.099999904632568
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 1.3; EcoRV--3.15; XbaI--3.15.
This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)
References

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

David J Stillman, personal communication

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet