pUK21-NotI (ATCC® 87800)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pUK21-NotI
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 3.2500000000000000
Intact vector size: 3.250
Type of vector: plasmid
Cloning sites: StuI XhoI HindIII BsiWI PstI SalI PmeI SacI EcoRI SfiI XbaI
Polylinker sites: SpeI StuI XhoI EcoRV NotI PvuII HindIII BsiWI PstI SalI PmeI
SacI EcoRV SpeI SfiI NotI XbaI SpeI ClaI
Construction: pUC21 and pFA6b
Features (with orientation and position when available):
Vector: pUK21-NotI (plasmid)
Construction: pUC21 and pFA6b
Construct size (kb): 3.25
cloning vector
Restriction digests of the clone give the following sizes (kb): EcoRV--3.25; XbaI--3.25; PvuI--2.0, 1.25.
This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment and insertion of the NotI polylinker fragment from pFA6b.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + kan)

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518

David J Stillman, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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