pUK21-NotI (ATCC® 87800)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pUK21-NotI
Depositors DJ Stillman
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 3.2500000000000000
DESCRIPTION OF VECTOR:
Intact vector size: 3.250
Type of vector: plasmid
Cloning sites: StuI XhoI HindIII BsiWI PstI SalI PmeI SacI EcoRI SfiI XbaI
Polylinker sites: SpeI StuI XhoI EcoRV NotI PvuII HindIII BsiWI PstI SalI PmeI
SacI EcoRV SpeI SfiI NotI XbaI SpeI ClaI
Construction: pUC21 and pFA6b
Features (with orientation and position when available):
Vector: pUK21-NotI (plasmid)
Construction: pUC21 and pFA6b
Marker(s):kanR
Construct size (kb): 3.25
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV--3.25; XbaI--3.25; PvuI--2.0, 1.25.
This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment and insertion of the NotI polylinker fragment from pFA6b.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + kan)
References

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518

David J Stillman, personal communication

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Shipping Information Distributed: freeze-dried
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