pEBA-4A8 (ATCC® 87790)

Applications: contains sequence  /  Depositors: EW Miles

Permits and Restrictions

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Designations pEBA-4A8
Depositors EW Miles
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 11.6400003433227500
Vector: pEBA-4A8 (plasmid)
Promoters: Promoter Ptrc
Construction: pEBA-10
Marker(s):TRP1,ampR
Construct size (kb): 11.64000034332275
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
Applications
contains sequence
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--11.6; EcoRI--5.5, 1.1 (5x); PstI--11.6; EcoRI/PstI--4.4; 1.1 (6x).
Overproduces alpha2 subunit of tryptophan synthase from Salmonella typhimurium.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

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