pEBA-4A8 (ATCC® 87790)

Applications: contains sequence  /  Depositors: EW Miles

Permits and Restrictions

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Designations pEBA-4A8
Depositors EW Miles
Biosafety Level 1
Vector Information
Size (kb): 11.6400003433227500
Vector: pEBA-4A8 (plasmid)
Promoters: Promoter Ptrc
Construction: pEBA-10
Marker(s):TRP1,ampR
Construct size (kb): 11.64000034332275
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
Applications
contains sequence
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--11.6; EcoRI--5.5, 1.1 (5x); PstI--11.6; EcoRI/PstI--4.4; 1.1 (6x).
Overproduces alpha2 subunit of tryptophan synthase from Salmonella typhimurium.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

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