Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Distribution host: Escherichia coli DH5
Size (kb): 7.4039998054504400
DESCRIPTION OF VECTOR:
Intact vector size: 7.404
Type of vector: plasmid
Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Features (with orientation and position when available):
replicon: ColE1, 1-362
coding sequence: bla, <-, 934-1794
replicon: ARS4, 2066-2442
centromere: CEN6, 2028-2053
marker(s): LEU2, <-, 3171-4265
promoter: PADH, 5666-6375
MCS: NdeI..PstI, ->, 6845-6899
ribosome-binding site: GAL4, ->, 6383-6791
transcription terminator: ADC1 transcriptional terminator (TADH), 6917-7107
signal peptide coding region: hemagglutinin epitope, 6797-6838
Vector: pC-ACT.2 (plasmid)
Promoters: Promoter PADH
Construct size (kb): 7.40399980545044
Features: marker(s): LEU2
ribosome-binding site: GAL4
signal peptide coding region: hemagglutinin epitope
transcription terminator: ADC1 transcriptional terminator (TADH)
coding sequence: bla
vector component of two-hybrid screening system
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; PstI--7.4; HindIII--7.4; NdeI--7.4.
Useful in two hybrid screening in yeast. A CEN based plasmid with Gal4 activation domain. It has lower copy number than 2 micron based plasmids.
Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5' GATC overhangs in all three reading frames.
Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464