pC-ACT.1 (ATCC® 87776)

Applications: cloning vectorshuttle vectorvector component of two-hybrid screening system  /  Depositors: PC Zambryski

Permits and Restrictions

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Designations pC-ACT.1
Depositors PC Zambryski
Biosafety Level 1
Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 7.3889999389648440
DESCRIPTION OF VECTOR:
Intact vector size: 7.389
Type of vector: plasmid
Cloning sites: NdeI SfiI NcoI SmaI BamHI SalI PstI
Polylinker sites: NdeI SfiI NcoI SmaI BamHI SalI PstI
Construction: pRS314
Features (with orientation and position when available):
replicon: ColE1, 1-362
coding sequence: bla, <-, 934-1794
replicon: ARS4, 2066-2442
centromere: CEN6, 2028-2053
marker(s): LEU2, <-, 3171-4265
promoter: PADH, 5666-6375
MCS: NdeI..PstI, ->, 6845-6884
ribosome-binding site: GAL4, ->, 6383-6791
transcription terminator: ADC1 transcriptional terminator (TADH), 6902-7092
signal peptide coding region: hemagglutinin epitope, 6797-6838
Vector: pC-ACT.1 (plasmid)
Promoters: Promoter PADH
Construction: pRS314
Marker(s):crbR
Construct size (kb): 7.388999938964844
Features: marker(s): LEU2
promoter: PADH
replicon: ARS4
replicon: ColE1
MCS: NdeI..PstI
ribosome-binding site: GAL4
signal peptide coding region: hemagglutinin epitope
transcription terminator: ADC1 transcriptional terminator (TADH)
centromere: CEN6
coding sequence: bla
Applications
cloning vector
shuttle vector
vector component of two-hybrid screening system
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; PstI--7.4; HindIII--7.4; NdeI--7.4.
Useful in two hybrid screening in yeast. A CEN based plasmid with Gal4 activation domain. It has lower copy number than 2 micron based plasmids.
Media Medium 1637: LB medium (ATCC medium 1065) with 100 mcg/ml carbenicillin
Growth Conditions
Temperature: 37.0°C
References

Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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