pC-ACT.1 (ATCC® 87776)

Applications: cloning vectorshuttle vectorvector component of two-hybrid screening system  /  Depositors: PC Zambryski

Permits and Restrictions

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Designations pC-ACT.1
Depositors PC Zambryski
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 7.3889999389648440
DESCRIPTION OF VECTOR:
Intact vector size: 7.389
Type of vector: plasmid
Cloning sites: NdeI SfiI NcoI SmaI BamHI SalI PstI
Polylinker sites: NdeI SfiI NcoI SmaI BamHI SalI PstI
Construction: pRS314
Features (with orientation and position when available):
replicon: ColE1, 1-362
coding sequence: bla, <-, 934-1794
replicon: ARS4, 2066-2442
centromere: CEN6, 2028-2053
marker(s): LEU2, <-, 3171-4265
promoter: PADH, 5666-6375
MCS: NdeI..PstI, ->, 6845-6884
ribosome-binding site: GAL4, ->, 6383-6791
transcription terminator: ADC1 transcriptional terminator (TADH), 6902-7092
signal peptide coding region: hemagglutinin epitope, 6797-6838
Vector: pC-ACT.1 (plasmid)
Promoters: Promoter PADH
Construction: pRS314
Marker(s):crbR
Construct size (kb): 7.388999938964844
Features: marker(s): LEU2
promoter: PADH
replicon: ARS4
replicon: ColE1
MCS: NdeI..PstI
ribosome-binding site: GAL4
signal peptide coding region: hemagglutinin epitope
transcription terminator: ADC1 transcriptional terminator (TADH)
centromere: CEN6
coding sequence: bla
Applications
cloning vector
shuttle vector
vector component of two-hybrid screening system
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; PstI--7.4; HindIII--7.4; NdeI--7.4.
Useful in two hybrid screening in yeast. A CEN based plasmid with Gal4 activation domain. It has lower copy number than 2 micron based plasmids.
Media ATCC® Medium 1637: LB medium (ATCC medium 1065) with 100 mcg/ml carbenicillin
Growth Conditions
Temperature: 37.0°C
References

Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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Product Sheet
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