pCu424CTR1-CYC1 (ATCC® 87734)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu424CTR1-CYC1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 6.2280001640319820
Vector: pCu424CTR1-CYC1 (phagemid)
Promoters: Promoter for expression CTR1-CYC1
Construction: pRS424, CTR1-CYC1 promoter
Marker(s):TRP1,ampR
Construct size (kb): 6.228000164031982
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--5.9, 0.4.
CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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