pCu425CUP1 (ATCC® 87728)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu425CUP1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 7.3359999656677250
Vector: pCu425CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS425, CUP1 promoter
Marker(s):LEU2,ampR
Construct size (kb): 7.335999965667725
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--7.3; SacI/SpeI--7.0, 0.26.
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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