pCu415CUP1 (ATCC® 87727)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu415CUP1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 6.5029997825622560
Vector: pCu415CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS415, CUP1 promoter
Marker(s):LEU2,ampR
Construct size (kb): 6.502999782562256
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CUP1
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
centromere: CEN6
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--6.5; SacI/SpeI--6.3, 0.26.
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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