pCu424CUP1 (ATCC® 87726)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu424CUP1
Depositors S Labbe, DJ Thiele
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.1030001640319820
Vector: pCu424CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS424, CUP1 promoter
Construct size (kb): 6.103000164031982
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
cloning vector
Restriction digests of the vector give the following sizes (kb): SacI--6.1; SacI/SpeI--5.9, 2.6.
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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