pCu424CUP1 (ATCC® 87726)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu424CUP1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 6.1030001640319820
Vector: pCu424CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS424, CUP1 promoter
Marker(s):TRP1,ampR
Construct size (kb): 6.103000164031982
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--6.1; SacI/SpeI--5.9, 2.6.
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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component of:ATCC 87778
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Attachments Vector map for 87726