pCu423CUP1 (ATCC® 87724)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu423CUP1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 6.2849998474121090
DESCRIPTION OF VECTOR:
Intact vector size: 6.285
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS423, CUP1 promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->
replicon: f1, <-
terminator: CYC1, <-
MCS: SpeI...XhoI, ->
promoter for expression: CUP1, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron, <-
Vector: pCu423CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS423, CUP1 promoter
Marker(s):ampR,HIS3
Construct size (kb): 6.284999847412109
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--6.3; SacI/SpeI--6.0, 0.26.
Gene CUP1 is the most potent for the copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

Related Products
component of:ATCC 87778
Shipped dried
Shipping Information Distributed: DNA supplied by depositor (frozen) (amount: 100 ng)
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