pSGP573 (ATCC® 87720)

Applications: contains sequence green fluorescent protein (GFP)  /  Depositors: J Hodson

Permits and Restrictions

View Permits

Designations pSGP573
Depositors J Hodson
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 9.3000001907348630
DESCRIPTION OF VECTOR:
Intact vector size: 9.300
Type of vector: plasmid
Cloning sites: XhoI SalI BamHI SmaI
Polylinker sites: XhoI SalI BamHI SmaI
Cloning capacity: Y
Construction: REP4X
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
coding sequence: GFP, ->
replicon: ars1
marker(s): ampR
marker(s): ura4+
promoter for expression: nmt, ->
MCS: XhoI...SmaI, ->
Vector: pSGP573 (plasmid)
Promoters: Promoter for expression nmt
Construction: REP4X
Marker(s):ampR
Construct size (kb): 9.300000190734863
Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt
replicon: ars1
MCS: XhoI...SmaI
coding sequence: GFP
Applications
contains sequence green fluorescent protein (GFP)
Comments
GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 7-720 of GFP matching nucleotides 21-734 of U87624, with additional modification S65T by changing codon to ACC.
The order of the major features in this plasmid is: nmt promotor - XhoI - ATG - GFP - GCT - NotI - SalI - BglII - SmaI - termination sequence - ars1 - pUC119 (3.3 kb) - ura4
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet