pSGP573 (ATCC® 87720)

Applications: contains sequence green fluorescent protein (GFP)  /  Depositors: J Hodson

Permits and Restrictions

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Designations pSGP573
Depositors J Hodson
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 9.300
Type of vector: plasmid
Cloning sites: XhoI SalI BamHI SmaI
Polylinker sites: XhoI SalI BamHI SmaI
Cloning capacity: Y
Construction: REP4X
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
coding sequence: GFP, ->
replicon: ars1
marker(s): ampR
marker(s): ura4+
promoter for expression: nmt, ->
MCS: XhoI...SmaI, ->
Vector: pSGP573 (plasmid)
Promoters: Promoter for expression nmt
Construction: REP4X
Marker(s): ampR
Construct size (kb): 9.30
Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt
replicon: ars1
MCS: XhoI...SmaI
coding sequence: GFP
Applications
contains sequence green fluorescent protein (GFP)
Comments
GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 7-720 of GFP matching nucleotides 21-734 of U87624, with additional modification S65T by changing codon to ACC.
The order of the major features in this plasmid is: nmt promotor - XhoI - ATG - GFP - GCT - NotI - SalI - BglII - SmaI - termination sequence - ars1 - pUC119 (3.3 kb) - ura4
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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