Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 5.1960000991821290
Vector: pSNAG (phagemid)
Promoters: Promoter CMV
Construction: pSCTEV, pSuperVSV
Construct size (kb): 5.196000099182129
Features: marker(s): ampR
other: Gal4p DNA-binding domain (DBD)
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40 ori
epitope tag: VSV-G
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2.
The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector.
Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205