pSNAG (ATCC® 87648)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: C Hovens

Permits and Restrictions

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Designations pSNAG
Depositors C Hovens
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.1960000991821290
Vector: pSNAG (phagemid)
Promoters: Promoter CMV
Construction: pSCTEV, pSuperVSV
Construct size (kb): 5.196000099182129
Features: marker(s): ampR
other: Gal4p DNA-binding domain (DBD)
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: SfuI...XbaI
terminator: SV40
epitope tag: VSV-G
expression vector
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2.
The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205

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