pSNAG (ATCC® 87648)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: C Hovens

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Designations pSNAG
Depositors C Hovens
Biosafety Level 1
Vector Information
Size (kb): 5.1960000991821290
Vector: pSNAG (phagemid)
Promoters: Promoter CMV
Construction: pSCTEV, pSuperVSV
Marker(s):ampR
Construct size (kb): 5.196000099182129
Features: marker(s): ampR
other: Gal4p DNA-binding domain (DBD)
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: SfuI...XbaI
terminator: SV40
epitope tag: VSV-G
Applications
expression vector
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2.
The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205

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component of:ATCC 87677
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