Size (kb): 5.1960000991821290
Vector: pSNAG (phagemid)
Promoters: Promoter CMV
Construction: pSCTEV, pSuperVSV
Construct size (kb): 5.196000099182129
Features: marker(s): ampR
other: Gal4p DNA-binding domain (DBD)
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40 ori
epitope tag: VSV-G
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2.
The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector.
Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205