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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.8000001907348630 Vector: pSLF104 (plasmid) Promoters: Promoter adh Marker(s):ampR,sup3-5 Construct size (kb): 4.800000190734863 Features: marker(s): ampR
marker(s): sup3-5
promoter: adh
repressor gene: tet
MCS: HindIII...EcoRI
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Applications
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YI-type (integrating) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.4, 0.58; BamHI--2.9, 1.9; EcoRI--3.6, 1.2. The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter. When integrated into a strain of genotype h- ade6-704 leu1-32 ura4-D18, the resulting fission yeast strain, FY191 (ATCC ) produces a constitutive tetracycline repressor protein.
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References
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Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516
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