pSLF104 (ATCC® 87621)

Applications: YI-type (integrating) shuttle vector  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations pSLF104
Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 4.8000001907348630
Vector: pSLF104 (plasmid)
Promoters: Promoter adh
Marker(s):ampR,sup3-5
Construct size (kb): 4.800000190734863
Features: marker(s): ampR
marker(s): sup3-5
promoter: adh
repressor gene: tet
MCS: HindIII...EcoRI
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.4, 0.58; BamHI--2.9, 1.9; EcoRI--3.6, 1.2.
The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter.
When integrated into a strain of genotype h- ade6-704 leu1-32 ura4-D18, the resulting fission yeast strain, FY191 (ATCC ) produces a constitutive tetracycline repressor protein.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

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