YCplac111 (ATCC® 87587)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Permits and Restrictions

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Designations YCplac111
Depositors RD Gietz
Biosafety Level 1
Vector Information
Size (kb): 6.112
Vector: YCplac111 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s): LEU2,ampR
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): BglII--6.5, EcoRV--6.5, BglI--4.7, 1.7.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

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Shipped frozen
Shipping Information Frozen E. coli containing the plasmid
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87587