pSON8 (ATCC® 87564)

Applications: vector component of two-hybrid screening system  /  Depositors: SJ Elledge

Permits and Restrictions

View Permits

Designations pSON8
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 9.4689998626708980
Vector: pSON8 (plasmid)
Promoters: Promoter ADH
Construction: pUC
Marker(s):URA3,ampR
Construct size (kb): 9.468999862670898
Features: marker(s): URA3
marker(s): ampR
promoter: ADH
replicon: 2 micron ori
replicon: pMB1
MCS: NcoI...EagI
terminator: ADH
coding sequence: SOS (human Son of sevenless)
Applications
vector component of two-hybrid screening system
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--6.0, 3.5; XbaI--9.5; BamHI--9.5.
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.
The vector contains the human SOS gene with a polylinker on the N-terminus to attach "bait" gene for use in SOS Recruitment System.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet