Size (kb): 10.0600004196167000
Vector: pLGdelta178 (plasmid)
Promoters: Promoter truncated CYC1 promoter (no UAS element)
Construct size (kb): 10.0600004196167
Features: marker(s): URA3
promoter: truncated CYC1 promoter (no UAS element)
replicon: 2 micron ori
coding sequence: lacZ
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): XhoI--11.0; HindIII--8.7, 2.2; EcoRI--4.75, 3.45, 2.4.
This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509
, 87510 & 87513). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity.
The vector was constructed by Lenny Guarente.
Guarente L, et al. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell 36: 503-511, 1984. PubMed: 6319028