pJL164 (ATCC® 87471)

Applications: YI-type (integrating) shuttle vectormarker deletion vector uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1  /  Depositors: JD Boeke

Permits and Restrictions

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Designations pJL164
Depositors JD Boeke
Biosafety Level 1
Host
Distribution host: Escherichia coli unknown (ATCC 33694)
Vector Information
Size (kb): 8.4
DESCRIPTION OF VECTOR:
Intact vector size: 8.400
Type of vector: phagemid
Cloning sites:
Polylinker sites:
Other unique sites: SmaI
Construction: pRS305
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, <-
replicon: f1, <-
other: URA3 deleter cassette
replicon: pMB1
marker(s): ampR, <-
Vector: pJL164 (phagemid)
Construction: pRS305
Marker(s):LEU2,ampR
Construct size (kb): 8.4
Features: marker(s): LEU2
marker(s): ampR
other: URA3 deleter cassette
replicon: f1
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
marker deletion vector uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--4.7, 4.0; XbaI--9.0; EcoRI--9.0.
ura3delta0 deletion hosts are generated by transformation with the SpeI/XhoI digested plasmid, pJL164, followed by selection on 5-FOA plates.
ura3 point mutation hosts are then differentiated from ura3delta0 deletion hosts by reversion analysis and Southern analysis.
This deleter vector is used to create designer yeast strains with a non-revertable ura3 auxotrophic marker deletion.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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component of:ATCC 87472
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