pLBT (ATCC® 87450)

Applications: suicide vectorvector permitting positive selection for transposon mutagenesisvector permitting visual detection of transposon mutagenesis beta-galactosidase beta-D-galactosidasevector that produces reporter gene fusion mutants via random transposon mutagenesis  /  Depositors: AE Goodman

Permits and Restrictions

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Designations pLBT
Depositors AE Goodman
Biosafety Level 1
Host
Distribution host: Escherichia coli SM10 lambda pir
Vector Information
Size (kb): 13.0900001525878900
DESCRIPTION OF VECTOR:
Intact vector size: 13.090
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: pLOFKm, pNK2804
Host range: Escherichia coli
Features (with orientation and position when available):
repressor gene: lacIq, <-
promoter: tac, ->
other: IS10R
other: mini-Tn10:lacZ:kanR
replicon: R6K
other: mobRP4
marker(s): ampR
Vector: pLBT (plasmid)
Promoters: Promoter tac
Construction: pLOFKm, pNK2804
Marker(s):ampR kanR
Construct size (kb): 13.09000015258789
Features: marker(s): ampR
other: IS10R
other: mini-Tn10:lacZ:kanR
other: mobRP4
promoter: tac
replicon: R6K
repressor gene: lacIq
Applications
suicide vector
vector permitting positive selection for transposon mutagenesis
vector permitting visual detection of transposon mutagenesis beta-galactosidase beta-D-galactosidase
vector that produces reporter gene fusion mutants via random transposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 4.6 (doublet); HindIII--8.0, 4.8, 1.1, 0.92; NotI--13.0, 1.7.
This vector was used to generate transposon reporter gene fusion mutants at high frequencies in Vibrio sp. S14 and Pseudomonas sp. S9. It may be a useful mutation analysis tool in other bacterial strains susceptible to Tn10 transposition.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Albertson NH, et al. Construction and use of a new vector/transposon pLBT::miniTn10:lac:kan, to identify environmentally responsive genes in a marine bacterium. FEMS Microbiol. Lett. 140: 287-294, 1996. PubMed: 8764492

Herrero M, et al. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172: 6557-6567, 1990. PubMed: 2172216

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Shipping Information Distributed: freeze-dried
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