pKKM13(+) (ATCC® 87403)

Applications: expression vectorvector permitting production of single-stranded DNAvector useful for site-directed mutagenesis  /  Depositors: R Kreutzer

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Designations pKKM13(+)
Depositors R Kreutzer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.9000000953674320
Vector: pKKM13(+) (phagemid)
Promoters: Promoter for expression tac
Marker(s):ampR
Construct size (kb): 4.900000095367432
Features: marker(s): ampR
promoter for expression: tac
replicon: M13
replicon: pMB1
MCS: EcoRI...HindIII
transcription terminator: T1
transcription terminator: T2
Applications
expression vector
vector permitting production of single-stranded DNA
vector useful for site-directed mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.6, 0.2; XbaI--4.8.
Expression vector useful for mutagenesis of cloned inserts.
Single stranded DNA obtained after superinfection with a suitable M13 helper phage gives the sense strand with respect to the tac promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nock S, et al. One-vector system for mutagenesis and over-expression of cloned genes. BioTechniques 18: 608, 1995. PubMed: 7598889

Roland Kreutzer, personal communication

Shipped frozen
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