These permits may be required for shipping this product:
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Size (kb): 5.4109997749328610
DESCRIPTION OF VECTOR:
Intact vector size: 5.411
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: SalI...XbaI, ->, 2271-2328
promoter for expression: CYC1, <-, 2329-2616
marker(s): ampR, <-, 3863-4793
replicon: CEN6/ARSH4, ->, 4887-5391
Vector: p416 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construct size (kb): 5.410999774932861
Features: marker(s): URA3
promoter for expression: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 0.3; KpnI--5.4; EcoRI--5.4.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504