p426 ADH (ATCC® 87377)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p426 ADH
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.4569997787475590
DESCRIPTION OF VECTOR:
Intact vector size: 7.457
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
promoter for expression: ADH, <-, 2339-3839
marker(s): ampR, <-, 4958-5888
replicon: 2 micron, ->, 5987-7327
Vector: p426 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS426 (ATCC 77107)
Marker(s):URA3,ampR
Construct size (kb): 7.456999778747559
Features: marker(s): URA3
marker(s): ampR
promoter for expression: ADH
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.8, 1.5; EcoRI--7.4; XbaI--4.6, 2.7.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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