p423 ADH (ATCC® 87371)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p423 ADH
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 7.529
Vector: p423 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS423
Marker(s):ampR,HIS3
Construct size (kb): 7.529
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: ADH
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.6; EcoRI--7.6; SacI/XbaI--3.2, 2.8, 1.5.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
High copy number shuttle expression vector.
This is a yeast high copy number shuttle expression vector with the 2 micron origin of replication, ADH promoter, HIS3 marker and CYC1 terminator.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
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