p416 TEF (ATCC® 87368)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Designations p416 TEF
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.5260000228881840
DESCRIPTION OF VECTOR:
Intact vector size: 5.526
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: p416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: XhoI...XbaI, ->, 2265-2328
promoter for expression: TEF, <-, 2329-2731
marker(s): ampR, <-, 3978-4908
replicon: CEN6/ARSH4, ->, 5012-5526
Vector: p416 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: p416
Marker(s):URA3,ampR
Construct size (kb): 5.526000022888184
Features: marker(s): URA3
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.6; SacI--uncut. The vector appears to have lost the SacI site.
Low copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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