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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.5260000228881840 DESCRIPTION OF VECTOR:
Intact vector size: 5.526
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: p416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: XhoI...XbaI, ->, 2265-2328
promoter for expression: TEF, <-, 2329-2731
marker(s): ampR, <-, 3978-4908
replicon: CEN6/ARSH4, ->, 5012-5526 Vector: p416 TEF (plasmid) Promoters: Promoter for expression TEF Construction: p416 Marker(s):URA3,ampR Construct size (kb): 5.526000022888184 Features: marker(s): URA3
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
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Applications
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YX-type (expression) shuttle vector vector with low copy number
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.6; SacI--uncut. The vector appears to have lost the SacI site. Low copy number shuttle expression vector.
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References
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Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
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Shipping Information
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Distributed: freeze-dried
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