p425 TEF (ATCC® 87367)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p425 TEF
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 7.482
Vector: p425 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS425
Marker(s):LEU2,ampR
Construct size (kb): 7.482
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: TEF
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.0, 2.4; SacI/XbaI--3.8, 3.2, 0.42.
High copy number shuttle expression vector.
this vector has the TEF promoter for expression, the LEU2 marker gene and the 2 micron replicon. It is distributed in E. coli.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information

 Freeze dried E. coli containing the plasmid

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