p416 GPD (ATCC® 87360)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Designations p416 GPD
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.8039999008178710
DESCRIPTION OF VECTOR:
Intact vector size: 5.804
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
replicon: CEN6/ARSH4, ->
marker(s): URA3, ->
terminator: CYC1, ->
promoter for expression: GPD, <-
marker(s): ampR, <-
Vector: p416 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS416
Marker(s):URA3,ampR
Construct size (kb): 5.803999900817871
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SstI/XbaI--5.13, 0.68; EcoRI--5.8, BamHI--5.8.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information Distributed: freeze-dried
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