p415 GALS (ATCC® 87346)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p415 GALS
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.6620001792907720
Vector: p415 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS415, GALS promoter
Marker(s):LEU2,ampR
Construct size (kb): 6.662000179290772
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GALS
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--4.2, 2.4; SacI/XbaI--6.2, 0.42.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped frozen
Shipping Information

 Freeze dried  E. coli containing the plasmid

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Attachments Vector map for 87346