p415 GALS (ATCC® 87346)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p415 GALS
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.6620001792907720
Vector: p415 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS415, GALS promoter
Construct size (kb): 6.662000179290772
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GALS
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--4.2, 2.4; SacI/XbaI--6.2, 0.42.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped frozen
Shipping Information

 Freeze dried  E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87346