p424 GALS (ATCC® 87345)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p424 GALS
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.2620000839233400
Vector: p424 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS424, GALS promoter
Marker(s):TRP1,ampR
Construct size (kb): 6.26200008392334
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GALS
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; XhoI--6.2; SacI/XbaI--3.2, 1.7, 1.0, 0.42.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped freeze-dried
Product Sheet
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Attachments Vector map for 87345