Size (kb): 6.2620000839233400
Vector: p424 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS424, GALS promoter
Construct size (kb): 6.26200008392334
Features: marker(s): TRP1
promoter for expression: GALS
replicon: 2 micron
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; XhoI--6.2; SacI/XbaI--3.2, 1.7, 1.0, 0.42.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736