Size (kb): 5.6110000610351560
Vector: p413 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS413, GALS promoter
Construct size (kb): 5.611000061035156
Features: marker(s): HIS3
promoter for expression: GALS
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.2, 0.4.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736