p415 GAL1 (ATCC® 87330)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p415 GAL1
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.7069997787475590
DESCRIPTION OF VECTOR:
Intact vector size: 6.707
Type of vector: plasmid
Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS415, GAL1 promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3451
promoter for expression: GAL1, <-, 3452-3912
marker(s): ampR, <-, 5159-6089
centromere: CEN6/ARSH4, ->, 6193-6707
Vector: p415 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS415, GAL1 promoter
Marker(s):LEU2,ampR
Construct size (kb): 6.706999778747559
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped frozen
Shipping Information Distributed: freeze-dried
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Attachments Vector map for 87330