Size (kb): 5.3949999809265140
Vector: p414 MET25 (plasmid)
Promoters: Promoter for expression MET25
Construction: pRS414, MET25 promoter
Construct size (kb): 5.394999980926514
Features: marker(s): TRP1
promoter for expression: MET25
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.7, 0.4; EcoRV--4.0, 1.4; HindIII--4.2, 1.2.
Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736