p414 MET25 (ATCC® 87320)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p414 MET25
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 5.3949999809265140
Vector: p414 MET25 (plasmid)
Promoters: Promoter for expression MET25
Construction: pRS414, MET25 promoter
Marker(s):TRP1,ampR
Construct size (kb): 5.394999980926514
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: MET25
MCS: XhoI-SpeI
terminator: CYC1
centromere: CEN6/ARSH4
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.7, 0.4; EcoRV--4.0, 1.4; HindIII--4.2, 1.2.
Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped freeze-dried
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Attachments Vector map for 87320